Jump to main content
Jump to site search

Issue 7, 2015
Previous Article Next Article

Micro-competition system for Raman quantification of multiple glycans on intact cell surface

Author affiliations

Abstract

A micro-competition system is designed for simultaneous quantification of multiple glycans on intact cell surfaces, by integrating two-surface–one-molecule competition with surface enhanced Raman scattering (SERS). The micro-competition is achieved among multiple-polysaccharide-coated gold nanostars functionalized silica bubbles, target cells and gold nanoprobes at a micron scale. The gold nanoprobes are prepared by coating distinct Raman molecules and lectins on gold nanoparticles for signal resolution and glycan recognition, respectively. The silica bubble surface serves as an artificial glycan surface and a SERS substrate. Upon the competitive recognition of lectin to the corresponding glycan, the gold nanoprobes can be specifically captured by the bubbles and cells in a homogeneous system, and the amounts of different gold nanoprobes on bubbles are simultaneously detected by SERS to reflect the corresponding glycan amounts on the cell surface. This micro-competition system with multiple quantification capability provides a powerful tool for investigation of the complex glycan-related biological processes.

Graphical abstract: Micro-competition system for Raman quantification of multiple glycans on intact cell surface

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Mar 2015, accepted on 30 Apr 2015 and first published on 30 Apr 2015


Article type: Edge Article
DOI: 10.1039/C5SC01031D
Author version
available:
Download author version (PDF)
Chem. Sci., 2015,6, 3769-3774
  • Open access: Creative Commons BY license
    All publication charges for this article have been paid for by the Royal Society of Chemistry

  •   Request permissions

    Micro-competition system for Raman quantification of multiple glycans on intact cell surface

    Y. Chen, L. Ding, J. Xu, W. Song, M. Yang, J. Hu and H. Ju, Chem. Sci., 2015, 6, 3769
    DOI: 10.1039/C5SC01031D

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements