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Issue 7, 2015
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Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

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Abstract

Selected new fluorogenic probes that interact in different ways with Hg2+ and MeHg+ have been prepared and used for the chemical speciation of both cations in aqueous solution as well as in HEK293 cells. The best selective speciation of Hg2+ and MeHg+ has been achieved by in vitro approaches based on fluorogenic probes supported in cultured cells, due to the particular sensitivity of the HEK293 cells to permeation by Hg2+, MeHg+ and the fluorogenic probes. In particular, MeHg+ was selectively detected in cell nuclei by probe JG45.

Graphical abstract: Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

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Supplementary files

Article information


Submitted
26 Feb 2015
Accepted
30 Apr 2015
First published
30 Apr 2015

This article is Open Access
All publication charges for this article have been paid for by the Royal Society of Chemistry

Chem. Sci., 2015,6, 3757-3764
Article type
Edge Article
Author version available

Chemical speciation of MeHg+ and Hg2+ in aqueous solution and HEK cells nuclei by means of DNA interacting fluorogenic probes

B. Díaz de Greñu, J. García-Calvo, J. Cuevas, G. García-Herbosa, B. García, N. Busto, S. Ibeas, T. Torroba, B. Torroba, A. Herrera and S. Pons, Chem. Sci., 2015, 6, 3757
DOI: 10.1039/C5SC00718F

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