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Issue 8, 2014
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Simultaneous fluorescent imaging of Cys/Hcy and GSH from different emission channels

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Abstract

A 4-methoxythiophenol-substituted pyronin dye 1 was exploited as reaction-type fluorescent probe for biothiols Cys/Hcy and GSH. The probe itself is nonfluorescent due to the photoinduced electron transfer (PET) process. The Cys (or Hcy)-induced substitution–rearrangement cascade reaction and GSH-induced substitution reaction with the probe lead to the corresponding aminopyronin and thiopyronin dyes with distinct photophysical properties, enabling Cys/Hcy and GSH to be detected from visible and near-infrared (NIR) emission channels, respectively, in pure PB buffer with relatively fast kinetics and obvious fluorescence turn-on response. Assisted by laser scanning confocal microscope, we also demonstrated that probe 1 could simultaneously sense Cys/Hcy and GSH in B16 cells in multicolor imaging.

Graphical abstract: Simultaneous fluorescent imaging of Cys/Hcy and GSH from different emission channels

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Publication details

The article was received on 21 Mar 2014, accepted on 24 Apr 2014 and first published on 24 Apr 2014


Article type: Edge Article
DOI: 10.1039/C4SC00838C
Citation: Chem. Sci., 2014,5, 3183-3188

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    Simultaneous fluorescent imaging of Cys/Hcy and GSH from different emission channels

    J. Liu, Y. Sun, H. Zhang, Y. Huo, Y. Shi and W. Guo, Chem. Sci., 2014, 5, 3183
    DOI: 10.1039/C4SC00838C

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