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Issue 10, 2013
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A new approach to light up DNA/Ag nanocluster-based beacons for bioanalysis

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Abstract

Dynamic DNA assembly, operated in an autonomous and reconfigurable manner by controlling the kinetics of strand displacement reactions (SDR), is an ideal approach to amplify the fluorescent signals for molecular diagnostic and imaging. Herein, we for the first time have demonstrated an enhancement of fluorescence intensity of DNA/Ag nanocluster-based beacons by the modulation of SDR. This is a new DNA/Ag NCs fluorescence light-up system through the use of the enhancer of G-rich overhang. Such a sensing system can be used to develop a DNA/Ag nanocluster-based beacon for the fluorescent detection of nucleic acid and thrombin with high selectivity and sensitivity, in which the detection sensitivity could be further enhanced through additional Exo III based amplification.

Graphical abstract: A new approach to light up DNA/Ag nanocluster-based beacons for bioanalysis

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Supplementary files

Article information


Submitted
10 May 2013
Accepted
10 Jul 2013
First published
10 Jul 2013

Chem. Sci., 2013,4, 4004-4010
Article type
Edge Article

A new approach to light up DNA/Ag nanocluster-based beacons for bioanalysis

L. Zhang, J. Zhu, Z. Zhou, S. Guo, J. Li, S. Dong and E. Wang, Chem. Sci., 2013, 4, 4004
DOI: 10.1039/C3SC51303C

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