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Issue 7, 2013
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Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

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Abstract

Dynamic protein assembly along supramolecular columnar polymers has been achieved through the site-specific covalent attachment of different SNAP-tag fusion proteins to self-assembled benzylguanine-decorated discotics. The self-assembly of monovalent discotics into supramolecular polymers creates a multivalent, bio-orthogonal and self-regulating framework for protein assembly. The intrinsic reversibility of supramolecular interactions results in reorganization and exchange of building blocks allowing for dynamic intermixing of protein-functionalized discotics between different self-assembled polymers, leading to self-optimization of protein arrangement and distance as evidenced by efficient energy transfer between fluorescent proteins.

Graphical abstract: Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

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Publication details

The article was received on 03 Apr 2013, accepted on 26 Apr 2013 and first published on 26 Apr 2013


Article type: Edge Article
DOI: 10.1039/C3SC50891A
Chem. Sci., 2013,4, 2886-2891
  • Open access: Creative Commons BY license
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    Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

    K. Petkau-Milroy, D. A. Uhlenheuer, A. J. H. Spiering, J. A. J. M. Vekemans and L. Brunsveld, Chem. Sci., 2013, 4, 2886
    DOI: 10.1039/C3SC50891A

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