Reactive protein-repellent surfaces for the straightforward attachment of small molecules up to whole cells

Abstract

Here we present a readily accessible strategy for creating protein-resistant surfaces that contain azides, based on the spontaneous adsorption of a comb-like copolymer. Using a strain-promoted azide alkyne cycloaddition, we illustrate the single-step conjugation of various representatives of important applications, being the labelling with small organic molecules, capture of nanoparticles, attachment of native enzymes at a controlled density, and finally control of cell adhesion. We thus demonstrate that advanced biomaterials can be created in two straightforward steps without requiring additional reagents, which should make this strategy a valuable tool for researchers in diverse fields.