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Issue 25, 2020
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Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme

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Abstract

Carbon quantum dots (CQDs) were synthesized from litchi peel, exhibiting a peroxidase-like activity and enabling the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in association with H2O2 to generate blue oxidized TMB (ox-TMB) with a strong absorption peak at 652 nm. Interestingly, the ox-TMB could be further reduced by ascorbic acid (AA) leading to fading of the blue color and an absorbance decrease. Thus, a convenient and sensitive colorimetric method for detection of AA using CQDs as peroxidase mimics was established. Several factors, such as acidity, temperature, incubating time, and TMB concentration, which might influence the response of the analysis signal, were optimized. The results showed that the decrease of absorbance (ΔA) was in good linear agreement with AA concentration in the range of 1.0–105 μM, with a low detection limit of 0.14 μM. The feasibility of this method was also investigated in commercial beverages with the 94.3–110.0% recovery.

Graphical abstract: Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme

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Article information


Submitted
05 Mar 2020
Accepted
01 Apr 2020
First published
16 Apr 2020

This article is Open Access

RSC Adv., 2020,10, 14953-14957
Article type
Paper

Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme

X. Shu, Y. Chang, H. Wen, X. Yao and Y. Wang, RSC Adv., 2020, 10, 14953
DOI: 10.1039/D0RA02105A

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