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Issue 53, 2019
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Nanopipette-assisted single cell metabolic glycan labeling

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Abstract

Here, we report a single cell glycan labeling strategy by combining nanoscale intracellular glass electrodes with bioorthogonal reaction. With the tip diameter less than 100 nm, the nanopipette electrode can be spatially controlled to inject artificial monosaccharides into single living cells with minimal invasion. The injection process can be precisely regulated by electroosmotic flow inside the nanopipette, and fluorescence labeling of sialic acid at single cell level is achieved.

Graphical abstract: Nanopipette-assisted single cell metabolic glycan labeling

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Supplementary files

Article information


Submitted
23 Aug 2019
Accepted
23 Sep 2019
First published
27 Sep 2019

This article is Open Access

RSC Adv., 2019,9, 30666-30670
Article type
Paper

Nanopipette-assisted single cell metabolic glycan labeling

Z. Zhou, X. Wang, J. Lv, B. Chen, Y. Tang and R. Qian, RSC Adv., 2019, 9, 30666
DOI: 10.1039/C9RA06634A

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