Issue 51, 2019, Issue in Progress
From the journal:

RSC Advances

To beat the heat – engineering of the most thermostable pyruvate decarboxylase to date†

Samuel Sutiono,a   Katharina Satzinger,‡a   André Pick,a   Jörg Carstenab  and  Volker Sieber*abcd  

* Corresponding authors

a Chair of Chemistry of Biogenic Resources, Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Schulgasse 16, 94315 Straubing, Germany
E-mail: sieber@tum.de

b Catalytic Research Center, Technical University of Munich, Ernst-Otto-Fischer-Straße 1, 85748 Garching, Germany

c Straubing Branch BioCat Fraunhofer IGB, Schulgasse 11a, 94315 Straubing, Germany

d School of Chemistry and Molecular Biosciences, The University of Queensland, 68 Copper Road, St. Lucia 4072, Australia

Abstract

Pyruvate decarboxylase (PDC) is a key enzyme for the production of ethanol at high temperatures and for cell-free butanol synthesis. Thermostable, organic solvent stable PDC was evolved from bacterial PDCs. The new variant shows >1500-fold-improved half-life at 75 °C and >5000-fold-increased half-life in the presence of 9 vol% butanol at 50 °C.

Graphical abstract: To beat the heat – engineering of the most thermostable pyruvate decarboxylase to date

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Article information

DOI
https://doi.org/10.1039/C9RA06251C
Article type
Paper
Submitted
11 Aug 2019
Accepted
03 Sep 2019
First published
20 Sep 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 29743-29746

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To beat the heat – engineering of the most thermostable pyruvate decarboxylase to date

S. Sutiono, K. Satzinger, A. Pick, J. Carsten and V. Sieber, RSC Adv., 2019, 9, 29743 DOI: 10.1039/C9RA06251C

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