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Issue 12, 2019
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Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers

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Abstract

Fluorescent immunosorbent assay (FIA) is very promising for sensitive and selective analysis in bio-medical applications. Here, we proposed an assay, using fluorescent engineering of analytes and the corresponding molecularly imprinted polymers (MIPs) as a plastic antibody. Three drug molecules (metronidazole, zidovudine and lamivudine) were condensed with 9-aminoacridine, using succinic anhydride as a spacer. The target products were characterized with 1H-NMR, IR and mass spectrometry. UV-vis absorption and fluorescent properties of the fluorophore-labeled drug molecules were investigated. Feasibility of the fluorescent biomimetic immunosorbent assay based on MIPs was demonstrated in the solution. This work will provide sound foundation for the future application in real sample.

Graphical abstract: Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers

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Supplementary files

Article information


Submitted
17 Jan 2019
Accepted
13 Feb 2019
First published
26 Feb 2019

This article is Open Access

RSC Adv., 2019,9, 6779-6784
Article type
Paper

Synthesis of fluorescent drug molecules for competitive binding assay based on molecularly imprinted polymers

M. Wubulikasimu, T. Muhammad, M. Imerhasan, N. Hudaberdi, W. Yang, J. Zhao and X. Peng, RSC Adv., 2019, 9, 6779
DOI: 10.1039/C9RA00422J

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