Issue 20, 2019, Issue in Progress

Dominance of Candidatus saccharibacteria in SBRs achieving partial denitrification: effects of sludge acclimating methods on microbial communities and nitrite accumulation

Abstract

Partial denitrification (NO3-N → NO2-N) was combined with anaerobic ammonium oxidation (ANAMMOX) to achieve nitrogen removal with a low C/N ratio and low energy consumption. Three different acclimation conditions, namely, R1 (sequencing batch reactor (SBR) under anoxic conditions), R2 (SBR under alternating anoxic/aerobic conditions), and R3 (SBR under low-intensity aeration), were investigated using glucose as an electron donor to achieve continuous accumulation of nitrite during a 120 d run. Subsequently, the denitrification performance and microbial community structure of the sludge were investigated. The results showed that the acclimatized sludge in reactors R2 and R3 achieved better partial denitrification performance than the sludge in R1 due to the presence of dissolved oxygen as a result of aeration. Notably, the R3 reactor had the optimal conditions for nitrite accumulation. The high-throughput sequencing analysis indicated that the dominant bacteria in R2 and R3 were Candidatus saccharibacteria with a relative abundance of 45.44% and 34.96%, respectively. This was the first time that Candidatus saccharibacteria was reported as the dominant bacteria in denitrifying sludge. The microbial diversity of the R1 reactor was much greater than that of R2 and R3, indicating that a larger proportion of denitrifying bacteria were present in the R2 and R3 reactors. In addition, the batch experiments showed that the higher the initial pH, the higher the nitrite accumulation rate was.

Graphical abstract: Dominance of Candidatus saccharibacteria in SBRs achieving partial denitrification: effects of sludge acclimating methods on microbial communities and nitrite accumulation

Article information

Article type
Paper
Submitted
19 Nov 2018
Accepted
01 Apr 2019
First published
10 Apr 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 11263-11271

Dominance of Candidatus saccharibacteria in SBRs achieving partial denitrification: effects of sludge acclimating methods on microbial communities and nitrite accumulation

W. Xiujie, W. Weiqi, Z. Jing, W. Siyu and L. Jun, RSC Adv., 2019, 9, 11263 DOI: 10.1039/C8RA09518C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements