Construction of myoglobin–amphiphilic alginate caprylamide–graphene composite modified electrode for the direct electron transfer between redox proteins and electrode and electrocatalysis of myoglobin

Abstract

To achieve the dispersion of the hydrophobic graphene (GR), the amphiphilic alginate caprylamide (ACA) was synthesized to fabricate electroactive Nafion/Mb–ACA–GR/CILE for the accurate determination of trichloroacetic acid (TCA). SEM observation, FT-IR and UV-Vis spectroscopic analysis indicated that ACA could tightly immobilize Mb and GR on the electrode surface by constructing biointerfaces, which not only provided Mb a suitable microenvironment to maintain its biological activity, but also shortened the distances between the active centers of Mb with carbon ionic liquid electrode (CILE), thus promoting the electron transfer rate. The electrochemical characterization of Nafion/Mb–ACA–GR/CILE showed that the direct electron transfer of Mb was realized on the modified electrode, which was attributed to the high electrical conductivity and excellent electrocatalytic activity of GR and good biocompatibility of ACA. Moreover, Nafion/Mb–ACA–GR/CILE exhibited good electrocatalytic activity towards TCA with the linear range from 2.5 to 47.3 mmol L⁻¹ and lower K_M^app value of 8.3 mmol L⁻¹. Moreover, the modified electrode also revealed good stability, reproducibility and accurate detection of tap-water, exhibiting great potential for the applications as the third-generation electrochemical biosensors.