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Issue 59, 2018, Issue in Progress
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Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

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Abstract

The properties of scaffolds for bone tissue engineering, including their biocompatibility, highly interconnected porosity, and mechanical integrity, are critical for promoting cell adhesion, proliferation, and osteoinduction. We used various physical and biological assays to obtain in vitro confirmation that the proposed composite scaffolds are potentially suitable for applications to bone tissue engineering. The proposed new composite scaffolds, which we fabricated by a rapid prototyping technique, were composed of mesoporous magnesium–calcium silicate (m_MCS), polycaprolactone (PCL), and polybutylene succinate (PBSu). We systematically evaluated the characteristics of the composite scaffolds, such as the hydrophilicity and bioactivity. We also investigated the proliferation and osteogenic differentiation of human mesenchymal stem cells (MSCs) scaffolded on the m_MCS/PCL/PBSu composite. Our results showed that, compared to the m_MCS/PCL scaffold, the m_MCS/PCL/PBSu scaffold has improved water absorption, in vitro degradability, biocompatibility, and bioactivity in simulated body fluid, while its mechanical strength is reduced. Moreover, the results of the cytotoxicity tests specified in ISO 10993-12 and ISO 10993-5 clearly indicate that the m_MCS/PCL scaffold is not toxic to cells. In addition, we obtained significant increases in initial cell attachment and improvements to the osteogenic MSC differentiation by replacing the m_MCS/PCL scaffold with the m_MCS/PCL/PBSu scaffold. Our results indicate that the m_MCS/PCL/PBSu scaffold achieves enhanced bioactivity, degradability, cytocompatibility, and osteogenesis. As such, this scaffold is a potentially promising candidate for use in stem cell-based bone tissue engineering.

Graphical abstract: Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

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Article information


Submitted
25 Jul 2018
Accepted
25 Sep 2018
First published
02 Oct 2018

This article is Open Access

RSC Adv., 2018,8, 33882-33892
Article type
Paper

Characterization and osteogenic evaluation of mesoporous magnesium–calcium silicate/polycaprolactone/polybutylene succinate composite scaffolds fabricated by rapid prototyping

Y. G. Kang, J. Wei, J. E. Kim, Y. R. Wu, E. J. Lee, J. Su and J. Shin, RSC Adv., 2018, 8, 33882
DOI: 10.1039/C8RA06281A

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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