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Issue 57, 2018
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Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

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Abstract

An aptamer-based fluorescence assay for culture-independent detection of Pseudomonas aeruginosa was developed. This assay was enabled by highly specific aptamers conjugated with photoluminescent carbon dots (CDs) as the fluorescent probe and graphene oxide (GO) as the quencher. Specially, high-throughput sequencing was achieved during systematic evolution of ligands via exponential enrichment (SELEX) for accurate recognition of aptamers. This assay displayed high specificity towards P. aeruginosa and was resistant to interference by other ubiquitous bacteria including Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis, and Clostridium perfringens. After the conditions were optimized, this assay achieved a wide detection range for P. aeruginosa varying from 101 CFU mL−1 to 107 CFU mL−1. Notably, it approached an excellent detection limit as low as 9 CFU mL−1. Therefore, this fluorescence assay was considered successfully developed for highly sensitive detection of P. aeruginosa. This assay also detected the contamination of P. aeruginosa in tap water and commercial bottled water, thereby suggesting its potential application in real water samples.

Graphical abstract: Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

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Supplementary files

Article information


Submitted
06 Jun 2018
Accepted
12 Sep 2018
First published
19 Sep 2018

This article is Open Access

RSC Adv., 2018,8, 32454-32460
Article type
Paper

Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

H. Wang, Z. Chi, Y. Cong, Z. Wang, F. Jiang, J. Geng, P. Zhang, P. Ju, Q. Dong and C. Liu, RSC Adv., 2018, 8, 32454
DOI: 10.1039/C8RA04819C

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