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Issue 27, 2018, Issue in Progress
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Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays

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Abstract

Pathogens infect hosts often through initial binding of their cell surface lectins to glycans expressed on the exterior of host cells. Thus, methods to evaluate the glycan-binding properties of pathogens are of great importance. Because of the multivalent nature of interactions of pathogens with glycans, the ability to assess the glycan density-dependent binding of pathogens is particularly important. In this study, we developed a facile technique to construct multivalent carbohydrate microarrays through immobilization of unmodified glycans on multivalent hydrazide-derivatized glass surfaces. This immobilization strategy does not require the use of multivalent glycoconjugates, which are typically prepared by using multistep sequences. The results of analysis of microarray images, obtained after incubation of multivalent glycan microarrays with cholera toxin B and pathogens such as uropathogenic E. coli and H. pylori, show that the binding affinities of toxins and pathogens for glycans are highly glycan density-dependent. Specifically, toxins and pathogens bind to glycans more strongly as the valency of the glycans on the microarrays is increased from 1 to 4. It is anticipated that the newly developed immobilization method will be applicable to the preparation of multivalent carbohydrate microarrays that are employed to evaluate multivalent glycan binding properties of a variety of pathogens and toxins.

Graphical abstract: Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays

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Publication details

The article was received on 09 Feb 2018, accepted on 15 Apr 2018 and first published on 19 Apr 2018


Article type: Paper
DOI: 10.1039/C8RA01285G
Citation: RSC Adv., 2018,8, 14898-14905
  • Open access: Creative Commons BY-NC license
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    Analysis of binding properties of pathogens and toxins using multivalent glycan microarrays

    H. S. Kim, J. Y. Hyun, S. Park and I. Shin, RSC Adv., 2018, 8, 14898
    DOI: 10.1039/C8RA01285G

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