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Issue 20, 2018, Issue in Progress
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Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

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Abstract

Quercetin spectrofluorometric quantification was carried out in aqueous media (pH 7) using micelles of surfactants, namely: CTAB, SDS and TX100 as promoters of quercetin fluorescence, having a critical micelle concentration, CMC, of: 0.94 ± 0.03, 7.7 ± 0.6 and 0.18 ± 0.3 mM, respectively, measured through UV-Vis spectrophotometry. The thermodynamic binding constant, K, for the quercetin-surfactants' micelles supramolecular complex was estimated to be (log(K/M−1)) = 2.87 ± 0.02, 2.78 ± 0.04 and 2.80 ± 0.04, respectively, from fluorescence spectrophotometry. With the aid of these parameters it was possible to construct species distribution diagrams and choose the experimental conditions where quercetin can be quantified in aqueous media from fluorescence measurements. The best lowest limit of detection (0.244 ± 0.092) μM was achieved with CTAB micelles while the best sensitivity (2.919 ± 0.054) M−1 corresponded to SDS.

Graphical abstract: Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

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Publication details

The article was received on 07 Feb 2018, accepted on 14 Mar 2018 and first published on 20 Mar 2018


Article type: Paper
DOI: 10.1039/C8RA01213J
RSC Adv., 2018,8, 10980-10986
  • Open access: Creative Commons BY-NC license
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    Quercetin spectrofluorometric quantification in aqueous media using different surfactants as fluorescence promoters

    J. C. Alva-Ensastegui, M. Palomar-Pardavé, M. Romero-Romo and M. T. Ramírez-Silva, RSC Adv., 2018, 8, 10980
    DOI: 10.1039/C8RA01213J

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