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Issue 74, 2017, Issue in Progress
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Rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification

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Abstract

We herein describe a rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification (eSDA). The developed strategy utilizes specially designed DNA probes that form mismatched thymine–thymine (T–T) base pairs at their 3′-ends. In the absence of Hg2+, the mismatched T–T base pairs prohibit the DNA polymerase-mediated extension and the subsequent eSDA does not occur. On the other hand, the presence of Hg2+ that mediates the formation of stable T–Hg2+–T base pairs, enables the extension of DNA probes by DNA polymerase. As a result, a large number of duplexes are produced through the eSDA, which is simply monitored in real-time by the gradual fluorescence enhancement from SYBR green I. By employing this strategy, we detected Hg2+ down to 2.95 pM within 30 min, and the practical applicability of this method was successfully demonstrated by detecting Hg2+ in tap water.

Graphical abstract: Rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification

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Publication details

The article was received on 21 Aug 2017, accepted on 29 Sep 2017 and first published on 06 Oct 2017


Article type: Paper
DOI: 10.1039/C7RA09226A
Citation: RSC Adv., 2017,7, 47143-47147
  • Open access: Creative Commons BY license
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    Rapid and label-free strategy for the sensitive detection of Hg2+ based on target-triggered exponential strand displacement amplification

    C. Y. Lee, H. Y. Kim, J. K. Ahn, K. S. Park and H. G. Park, RSC Adv., 2017, 7, 47143
    DOI: 10.1039/C7RA09226A

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