Jump to main content
Jump to site search

Issue 33, 2017

Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

Author affiliations

Abstract

Bacillus mojavensis JF-2 produces water-soluble lipopeptide under aerobic conditions, while Pseudomonas stutzeri DQ1 grows rapidly under anaerobic conditions. These bacteria were used to construct an engineered strain for anaerobic lipopeptide production by protoplast fusion for potential use in microbially enhanced oil recovery (MEOR). The resulting fusant strain FA-2 produced lipopeptide (382 mg l−1) anaerobically at temperatures up to 50 °C, across a pH range of 4.5–10.0, and at salt concentrations as high as 10% NaCl. Experimental results from a physical simulation core at 39 °C suggest that FA-2 has potential for use in MEOR.

Graphical abstract: Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

Article information


Submitted
27 Feb 2017
Accepted
03 Apr 2017
First published
10 Apr 2017

This article is Open Access

RSC Adv., 2017,7, 20667-20676
Article type
Paper

Anaerobic lipopeptide biosurfactant production by an engineered bacterial strain for in situ microbial enhanced oil recovery

X. Liang, R. Shi, M. Radosevich, F. Zhao, Y. Zhang, S. Han and Y. Zhang, RSC Adv., 2017, 7, 20667 DOI: 10.1039/C7RA02453C

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements