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Issue 24, 2017
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A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

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Abstract

Enzyme kinetics and classification are key topics in modern biochemistry. Here, we have developed a simple, rapid, and quantitative assay via a terminally capped DNA probe, reaction-specific-labeling and the fluorescence-quenching capability of graphene-oxide. Our technique provides not only characterization of kinetic information but also the immediate classification of an exonuclease without needing to design a set of DNA substrates.

Graphical abstract: A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

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Supplementary files

Article information


Submitted
10 Jan 2017
Accepted
03 Mar 2017
First published
06 Mar 2017

This article is Open Access

RSC Adv., 2017,7, 14917-14922
Article type
Paper

A graphene oxide-based tool-kit capable of characterizing and classifying exonuclease activities

J. Song, V. M. Hoa, J. Yoo, S. Oh, H. Im, D. Park and G. Lee, RSC Adv., 2017, 7, 14917
DOI: 10.1039/C7RA00388A

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