Issue 31, 2017, Issue in Progress

Colorimetric detection of cholic acid based on an aptamer adsorbed gold nanoprobe

Abstract

Cholic acid (CA) has been proved as an effective clinical biomarker for disease diagnosis and treatment monitoring. However, traditional CA detection methods are tedious, time-consuming and expensive, and the results cannot be visualized. Herein, we present a facile, low-cost, fast and label-free biosensor platform based on a CA aptamer (48 nt) modified gold nanoprobe for CA detection. CA can bind with the surface-adsorbed CA aptamers competitively and detach the aptamer from the gold nanoparticles (Au NPs). Au NPs modified with aptamers of different densities will grow into morphologically varied nanostructures. The result can be observed visibly and quantified easily with the localized surface plasmon resonance (LSPR) spectra. The detection limit (LOD) was 1 μM, which was lower than the existing colorimetric detection methods based on Au NPs. Furthermore, the affinity between CA and 48 nt aptamer was verified for the first time by MicroScale Thermophoresis (MST) technology.

Graphical abstract: Colorimetric detection of cholic acid based on an aptamer adsorbed gold nanoprobe

Supplementary files

Article information

Article type
Paper
Submitted
07 Jan 2017
Accepted
12 Mar 2017
First published
31 Mar 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 19250-19256

Colorimetric detection of cholic acid based on an aptamer adsorbed gold nanoprobe

Q. Zhu, T. Li, Y. Ma, Z. Wang, J. Huang, R. Liu and Y. Gu, RSC Adv., 2017, 7, 19250 DOI: 10.1039/C7RA00255F

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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