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Issue 115, 2016, Issue in Progress
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Highly sensitive and selective fluorescence detection of Hg(II) ions based on R-phycoerythrin from Porphyra yezoensis

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Abstract

R-Phycoerythrin (R-PE) is a kind of natural fluorescent protein from marine Porphyra yezoensis. A highly sensitive and selective fluorimetric method has been developed to detect Hg2+ ions utilizing R-PE as a fluorescent probe. R-PE could respond selectively to Hg2+ ions among a series of metal ions. The reaction mechanism was investigated through UV-vis, fluorescence microscope imaging and fluorescence measurements. It was demonstrated that the interaction between the R-PE fluorescent probe and Hg2+ ions would cause fluorescence quenching. Besides, there was a linear relationship between R-PE fluorescence intensities and Hg2+ concentrations. A novel method was thus explored to detect Hg2+ ions with high sensitivity, selectivity, and a broad linear range of 0.0010–25.0 μM, which could allow for Hg2+ ions down to 0.0130 μM with a relative standard deviation of about 1.60%. Additionally, the method was successfully applied to detect Hg2+ ions in diverse water samples, showing the appropriate rates of recovery between 92.0% and 108.0%. This highly sensitive and selective natural green fluorescent probe of R-PE should have huge potential applications for detecting Hg2+ ions in diverse environments.

Graphical abstract: Highly sensitive and selective fluorescence detection of Hg(ii) ions based on R-phycoerythrin from Porphyra yezoensis

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Publication details

The article was received on 29 Sep 2016, accepted on 05 Dec 2016 and first published on 06 Dec 2016


Article type: Paper
DOI: 10.1039/C6RA24185A
RSC Adv., 2016,6, 114685-114689

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    Highly sensitive and selective fluorescence detection of Hg(II) ions based on R-phycoerythrin from Porphyra yezoensis

    Q. Wang, Y. Xu, Y. Hou, Y. Wang, M. Yan, X. Zhang and H. Wang, RSC Adv., 2016, 6, 114685
    DOI: 10.1039/C6RA24185A

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