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Issue 109, 2016, Issue in Progress
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A bio-hybrid material for adsorption and degradation of phenanthrene: bacteria immobilized on sawdust coated with a silica layer

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Abstract

Cell immobilization technology has been considered as an effective method for bioremediation of hydrocarbon-contaminated soil. However, bacteria immobilized by a single method often encounter some problems, e.g., cell leakage, cellular damage and no reproduction. In this study, a biomimetic hybrid material was constructed by pre-immobilization of bacteria on sawdust followed by coating a silica layer through vapor deposition (Silica-IC). The viability and metabolic activity of Silica-IC were investigated. Results showed that the silica layer covering the bacterial agent could significantly reduce cell leakage from sawdust without losing reproductive capacity on nutrient plates. A viability assay by SYTO9/PI in flow cytometry indicated that the proportion of live cells was decreased 30% and injured cells was increased 23.9%, while that of dead cells was still below 2.5% during storage at 4 °C for 15 days, i.e., membrane permeability of Silica-IC was increased, indicating bacterial cells in Silica-IC were able to maintain long-term storage stability and shelf life. The metabolic activity of Silica-IC toward phenanthrene (Phe) was enhanced both in liquid and soil. Phe degradation kinetics of Silica-IC in liquid medium well fitted an adsorption–degradation model, suggesting that the silica layer did not inhibit Phe diffusion. Moreover, the Phe removal percentage of Silica-IC in soil was up to 93.4% on day 2. Silica-IC in soil grew well and the growth was closely related to the residual amount of Phe. This work provides a route to develop a wide range of bio-materials for bioremediation.

Graphical abstract: A bio-hybrid material for adsorption and degradation of phenanthrene: bacteria immobilized on sawdust coated with a silica layer

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Article information


Submitted
11 Sep 2016
Accepted
03 Nov 2016
First published
03 Nov 2016

RSC Adv., 2016,6, 107189-107199
Article type
Paper

A bio-hybrid material for adsorption and degradation of phenanthrene: bacteria immobilized on sawdust coated with a silica layer

J. Li, C. Guo, C. Liao, M. Zhang, X. Liang, G. Lu, C. Yang and Z. Dang, RSC Adv., 2016, 6, 107189
DOI: 10.1039/C6RA22683C

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