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Issue 67, 2016
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Design of a core–shell support to improve lipase features by immobilization

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Abstract

Different core–shell polymeric supports, exhibiting different morphologies and composition, were produced through simultaneous suspension and emulsion polymerization, using styrene (S) and divinylbenzene (DVB) as co-monomers. Supports composed of polystyrene in both the core and the shell (PS/PS) and the new poly(styrene-co-divinylbenzene) support (PS-co-DVB/PS-co-DVB) were used for the immobilization of three different lipases (from Rhizomucor miehie (RML), from Themomyces lanuginosus (TLL) and the form B from Candida antarctica, (CALB)) and of the phospholipase Lecitase Ultra (LU). The features of the new biocatalysts were evaluated and compared to the properties of commercial biocatalysts (Novozym 435 (CALB), Lipozyme RM IM and Lipozyme TL IM) and biocatalysts prepared by enzyme immobilization onto commercial octyl-agarose, a support reported as very suitable for lipase immobilization. It was shown that protein loading and stability of the biocatalysts prepared with the core–shell supports were higher than the ones obtained with commercial octyl-agarose or the commercial lipase preparations. Besides, it was shown that the biocatalysts prepared with the core–shell supports also presented higher activities than commercial biocatalysts when employing different substrates, encouraging the use of the produced core–shell supports for immobilization of lipases and the development of new applications.

Graphical abstract: Design of a core–shell support to improve lipase features by immobilization

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Supplementary files

Article information


Submitted
23 May 2016
Accepted
20 Jun 2016
First published
27 Jun 2016

RSC Adv., 2016,6, 62814-62824
Article type
Paper
Author version available

Design of a core–shell support to improve lipase features by immobilization

E. A. Manoel, M. Pinto, J. C. S. dos Santos, V. G. Tacias-Pascacio, D. M. G. Freire, J. C. Pinto and R. Fernandez-Lafuente, RSC Adv., 2016, 6, 62814
DOI: 10.1039/C6RA13350A

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