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Issue 75, 2016
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Novel nonplanar and rigid fluorophores with intensive emission in water and the application in two-photon imaging of live cells

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Abstract

Bright and photostable fluorophores play a key role in the imaging of specific intracellular molecules. Here we report a facile approach to improve the brightness of naphthalimide fluorophores in water. We synthesized a series of nonplanar and rigid compounds (FM1, FM2, and FM3) by fusing a seven-membered heterocycle onto a naphthalimide skeleton. Compared with classic naphthalimide (FM0), these novel fluorophores exhibited much higher quantum yield values (Φ = 0.60–0.66), and higher molar extinction coefficients in aqueous solution, which elicited superior brightness to FM0. They also showed good photostability, pH-independence, and two-photon microscope (TPM) imaging ability. This new strategy of structural nonplanarity and rigidification provides an efficient way to improve the quantum efficiencies of traditional dyes.

Graphical abstract: Novel nonplanar and rigid fluorophores with intensive emission in water and the application in two-photon imaging of live cells

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Publication details

The article was received on 21 May 2016, accepted on 22 Jul 2016 and first published on 22 Jul 2016


Article type: Communication
DOI: 10.1039/C6RA13226J
RSC Adv., 2016,6, 71624-71627

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    Novel nonplanar and rigid fluorophores with intensive emission in water and the application in two-photon imaging of live cells

    J. Zhang, C. Wang, L. Zhang, H. Wu, Y. Xiao, Y. Xu, X. Qian and W. Zhu, RSC Adv., 2016, 6, 71624
    DOI: 10.1039/C6RA13226J

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