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Issue 67, 2016, Issue in Progress
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In vitro imaging of β-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

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Abstract

To evaluate the potential of monoolein-based cubic bicontinuous liquid crystalline nanoparticles (cubosomes) as diagnostic tools in diabetes and pancreatic β-cell transplantation, fluorescent cubosomes were formulated, entrapping within the lipid bilayer a newly synthesized, potent, hydrophobic dye. Cryo-TEM, SAXS, and DLS were performed to assess the morphological and structural aspects of this formulation. Rat pancreatic β-cells (INS-1E) readily took up the cubosome nanoparticles, as revealed by in vitro internalization tests, and flow-cytometric analyses confirmed the persistence of the fluorescence for up to 24 hours. The cytotoxicity of cubosomes at various concentrations, evaluated with a real-time analysis of proliferation, revealed that the proliferation curves relative to the less concentrated formulation (5 μM in terms of monoolein content) were comparable to the untreated cultures. INS-1E cell cycle and apoptosis supported such results. In conclusion, this formulation, with dye content in the nanomolar range, seems to be useful for β-cell imaging.

Graphical abstract: In vitro imaging of β-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

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Publication details

The article was received on 13 Apr 2016, accepted on 21 Jun 2016 and first published on 23 Jun 2016


Article type: Paper
DOI: 10.1039/C6RA09616F
RSC Adv., 2016,6, 62119-62127

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    In vitro imaging of β-cells using fluorescent cubic bicontinuous liquid crystalline nanoparticles

    V. Miceli, V. Meli, M. Blanchard-Desce, T. Bsaibess, M. Pampalone, P. G. Conaldi, C. Caltagirone, M. Obiols-Rabasa, J. Schmidt, Y. Talmon, A. Casu and S. Murgia, RSC Adv., 2016, 6, 62119
    DOI: 10.1039/C6RA09616F

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