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Issue 17, 2016
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Hydrophobic chain modified low molecular weight polyethylenimine for efficient antigen delivery

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Abstract

The development of new therapeutic vaccines for the efficient induction of cellular immunity by antigen cross-presentation in antigen-presenting cells, especially dendritic cells, is regarded as a promising approach to prime antigen-specific T cell responses and to destroy tumor cells. We synthesized low molecular weight polyethylenimine (C12-PEI) with a modified hydrophobic lipid chain by the direct coupling of 1-bromododecane with low molecular weight polyethylenimine (MW 423 Da) in a 2 : 1 molar ratio. This was bound with model antigen ovalbumin (OVA) to form sphere-like polyplex nanoparticles (size 158–302 nm; zeta potential −18.4 to −13.1 mV) by electrostatic interaction at an C12-PEI/OVA weight ratio of 0.03–0.08. The in vitro cytotoxicity of the C12-PEI/OVA nanoparticles was strongly dependent on the weight ratio of the C12-PEI and OVA. The antigen cross-presentation effect (IL-2 secretion) of the C12-PEI/OVA polyplexes was also dependent on the weight ratio. Optimized cross-presentation was achieved on the C12-PEI/OVA-0.07/1 polyplexes, which was 5.3-fold higher than that of the free OVA solution and 1.9-fold higher than that of the PEI/OVA polyplexes. The intracellular distribution of the C12-PEI/rhodamine-labeled OVA polyplexes demonstrated the lysosome localization effect. These results indicated that the synthesized C12-PEI cationic polymer with a modified hydrophobic lipid chain could potentially be used as a therapeutic vaccine carrier for delivery of the OVA antigen.

Graphical abstract: Hydrophobic chain modified low molecular weight polyethylenimine for efficient antigen delivery

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Supplementary files

Article information


Submitted
05 Dec 2015
Accepted
22 Jan 2016
First published
26 Jan 2016

RSC Adv., 2016,6, 13636-13643
Article type
Paper

Hydrophobic chain modified low molecular weight polyethylenimine for efficient antigen delivery

H. Wang, J. Chen, J. Ying, Y. Xu and R. Sheng, RSC Adv., 2016, 6, 13636
DOI: 10.1039/C5RA25919C

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