Issue 8, 2016

Cellulose as an efficient matrix for lipase and transaminase immobilization

Abstract

Immobilization of enzymes is important to improve their stability and to facilitate their recyclability, aiming to make biocatalytic processes more efficient. One of the important aspects is the utilization of cheap, abundant, and environmentally friendly carriers for enzyme immobilization. Here we report the use of functionalized cellulose for lipase and transaminase immobilization. High immobilization efficiencies (up to 90%) could be achieved for the transaminase from Vibrio fluvialis. For immobilized lipase CAL-B as well as the transaminase, good conversions and recyclability could be demonstrated in kinetic resolutions to afford chiral alcohols or amines. Moreover, such application of the immobilized transaminase enabled very high conversions in a continuous-flow process in the asymmetric synthesis of (S)-phenylethylamine (80% conversion, >99% ee).

Graphical abstract: Cellulose as an efficient matrix for lipase and transaminase immobilization

Supplementary files

Article information

Article type
Paper
Submitted
24 Nov 2015
Accepted
14 Dec 2015
First published
17 Dec 2015

RSC Adv., 2016,6, 6665-6671

Author version available

Cellulose as an efficient matrix for lipase and transaminase immobilization

S. P. de Souza, I. I. Junior, G. M. A. Silva, L. S. M. Miranda, M. F. Santiago, F. Leung-Yuk Lam, A. Dawood, U. T. Bornscheuer and R. O. M. A. de Souza, RSC Adv., 2016, 6, 6665 DOI: 10.1039/C5RA24976G

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