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Issue 5, 2016
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Ultrasensitive electrochemical detection of UO22+ based on DNAzyme and isothermal enzyme-free amplification

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Abstract

A novel strategy for ultrasensitive detection of UO22+ was proposed by using a UO22+-specific DNAzyme and two free labeled DNA hairpins. The principle and detection processes of this electrochemical biosensor for amplification detection of UO22+ were described. The detection conditions of the biosensor, such as the concentration of hairpins, pH, and the incubation time of HCR, were optimized. Under optimal conditions, this biosensor demonstrated a high sensitivity of UO22+ detection with the dynamic concentration range spanning from 0.05 to 4 nM and a detection limit of 20 pM at the 3Sblank level, providing a promising strategy for on site and real-time monitoring of uranium.

Graphical abstract: Ultrasensitive electrochemical detection of UO22+ based on DNAzyme and isothermal enzyme-free amplification

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Supplementary files

Article information


Submitted
30 Oct 2015
Accepted
07 Dec 2015
First published
18 Dec 2015

RSC Adv., 2016,6, 3960-3966
Article type
Paper

Ultrasensitive electrochemical detection of UO22+ based on DNAzyme and isothermal enzyme-free amplification

W. Yun, J. Jiang, D. Cai, X. Wang, G. Sang, J. Liao, T. Lu and K. Yan, RSC Adv., 2016, 6, 3960
DOI: 10.1039/C5RA22773A

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