Issue 10, 2016

Qualitative analysis of chiral alanine by UV-visible-shortwave near infrared diffuse reflectance spectroscopy combined with chemometrics

Abstract

UV-visible-shortwave near infrared diffuse reflectance spectroscopy (UV-vis-SWNIR DRS) combined with chemometrics is investigated for the first time to discriminate enantiomers and their racemic compounds, using D-, L- and DL-alanine as model compounds. After optimizing the measuring conditions of powder particle sizes and the distance between fiber probe and sample, discriminant partial least squares (PLS-DA) models were built with UV-vis-SWNIR DRS to implement the qualitative analysis of alanine chirality. As a result, under the optimized conditions of particle size sifted through 100-mesh and a distance of 5.3 mm, an excellent discrimination of chirality with an accuracy of 100% was obtained, which is better than that of manufacturers with an accuracy of 86.67%. The results of this study infer that UV-vis-SWNIR DRS combined with chemometrics can be a rapid, simple and noninvasive method for chiral analysis.

Graphical abstract: Qualitative analysis of chiral alanine by UV-visible-shortwave near infrared diffuse reflectance spectroscopy combined with chemometrics

Article information

Article type
Paper
Submitted
27 Oct 2015
Accepted
06 Jan 2016
First published
12 Jan 2016

RSC Adv., 2016,6, 8395-8405

Author version available

Qualitative analysis of chiral alanine by UV-visible-shortwave near infrared diffuse reflectance spectroscopy combined with chemometrics

X. Li, K. Xu, H. Li, S. Yao, Y. Li and B. Liang, RSC Adv., 2016, 6, 8395 DOI: 10.1039/C5RA22488H

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