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Issue 11, 2016
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Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells

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Abstract

In this study, we investigated the influence of processing methods on the chemo-physical properties of silk fibroin (SF) film and on their interaction with neural cells. Structural, thermal and morphological analysis revealed a strong correlation between the conformation, stability and texture of silk films and the fabrication conditions. An increase in temperature, methanol treatment and the use of a microfluidic approach led to an improvement in SF film stability in terms of β-sheet content, mechanical resistance, dissolution and enzymatic degradation. An effect on the interaction of SF films with neural cells, through a modulation of the surface properties, was also observed. In particular, hydrophobic surfaces induce proliferation of astrocytes and neuron adhesion whereas hydrophilic surfaces promote a remarkable neurite outgrowth. A detailed knowledge of the effect of manufacturing parameters on SF film properties can facilitate and extend the applications of silk-based biomaterials in tissue engineering and drug release systems.

Graphical abstract: Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells

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Supplementary files

Article information


Submitted
06 Oct 2015
Accepted
04 Jan 2016
First published
07 Jan 2016

RSC Adv., 2016,6, 9304-9314
Article type
Paper

Effect of different fabrication methods on the chemo-physical properties of silk fibroin films and on their interaction with neural cells

A. Sagnella, A. Pistone, S. Bonetti, A. Donnadio, E. Saracino, M. Nocchetti, C. Dionigi, G. Ruani, M. Muccini, T. Posati, V. Benfenati and R. Zamboni, RSC Adv., 2016, 6, 9304
DOI: 10.1039/C5RA20684G

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