Lipase immobilization towards improved productivity on kinetic resolutions by a continuous-flow process

Abstract

Development of asymmetric transformations for the synthesis of chiral molecules has achieved great results in the last decade. Besides the great evolution achieved during recent years in the biotechnology and biocatalysis fields, industrial processes using enzymatic approaches are few and a more broad application of such technology towards the synthesis of chiral molecules is still under development. Herein we report our results on the immobilization of lipase B from Candida antarctica on five different commercial supports for the development of a continuous-flow kinetic resolution of alcohol. Immobilization on Accurel MP1000 gave the best results arriving at productivities of around 140 g per h per g of protein and the immobilized biocatalyst could be recycled 5 times without significant loss of activity.