Facile synthesis of FeIII–tannic acid film-functionalized magnetic silica microspheres for the enrichment of low-abundance peptides and proteins for MALDI-TOF MS analysis†
In this work, novel FeIII–tannic acid (TA) film-functionalized magnetic silica microspheres (designated Fe3O4@SiO2@FTA) were prepared via a facile deposition of one-step assembled FeIII–TA films on the surface of magnetic silica microspheres. The resulting Fe3O4@SiO2@FTA microspheres possessed strong magnetic responsiveness and good dispersity in aqueous solutions. We introduced low concentrations of standard peptide, standard protein, peptides in BSA tryptic digests and human urine to investigate the enrichment capability of the prepared nanocomposites. The enrichment process was very fast (only 5 min) and the peptide/protein–magnetic microsphere conjugates were readily separated by an external magnet. Moreover, the obtained conjugates can be directly analyzed by MALDI-TOF MS without any elution procedure. The enrichment performance was quite satisfying, indicating Fe3O4@SiO2@FTA microspheres as a universally applicable material for efficient and fast enrichment of peptides and proteins.