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Issue 47, 2015
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Layer-by-layer assembled protein nanotubes with high DNA affinity

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Abstract

In this research, protein nanotubes with high affinity for DNA molecules were prepared by alternate layer-by-layer (LbL) assembly of human serum albumin (HSA) and polyethylenimine (PEI). The well-defined hollow cylinder (PEI/HSA)5PEI nanotubes with outer diameter 455 ± 13 nm, inner diameter 275 ± 35 nm, wall thickness 151 ± 7 nm and length 5.6 ± 0.9 μm were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and energy dispersive spectroscopy (EDS). Under optimal conditions, the maximum adsorption capacity of protein nanotubes for polyA25 was 606.0 mg g−1, which was far higher than the reported nanoparticles. The rates of adsorption were found to conform to the pseudo-second-order kinetics and intra-particle diffusion models with good correlation. The equilibrium adsorption data fitted well with the Langmuir isotherm model. The DNA release from DNA/(PEI/HSA)5PEI nanotubes was associated with the ionic strength and pH in the solution.

Graphical abstract: Layer-by-layer assembled protein nanotubes with high DNA affinity

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Publication details

The article was received on 17 Mar 2015, accepted on 16 Apr 2015 and first published on 16 Apr 2015


Article type: Paper
DOI: 10.1039/C5RA04725K
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RSC Adv., 2015,5, 37130-37137

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    Layer-by-layer assembled protein nanotubes with high DNA affinity

    P. Jiao, Y. Guo, A. Niu and X. Kang, RSC Adv., 2015, 5, 37130
    DOI: 10.1039/C5RA04725K

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