Jump to main content
Jump to site search

Issue 53, 2015
Previous Article Next Article

Lanthanum breaks the balance between osteogenesis and adipogenesis of mesenchymal stem cells through phosphorylation of Smad1/5/8

Author affiliations

Abstract

In the presence of lanthanum (La), the balance between osteogenesis and adipogenesis was broken in mouse mesenchymal stem cells (MSCs) in vitro, resulting in osteogenic induction and adipogenic inhibition, which were verified by alkaline phosphatase (ALP) activity and calcium secretion, and oil red O staining assays, respectively. The molecular data revealed that La up-regulated the expression levels of osteogenesis-associated genes and proteins, while it inhibited the expression of adipogenesis-associated genes and proteins. Furthermore, the mechanism of La on bone metabolism was investigated by screening 84 genes involved in differentiation of MSCs. The results showed that Smad1/5/8, a key protein involved in the bone morphogenetic protein (BMP) signaling pathway, was significantly phosphorylated under the exposure of La, leading to the activation of the BMP signaling pathway with subsequent up-regulation of the osteogenic genes and the down-regulation of the adipogenic genes.

Graphical abstract: Lanthanum breaks the balance between osteogenesis and adipogenesis of mesenchymal stem cells through phosphorylation of Smad1/5/8

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Feb 2015, accepted on 05 May 2015 and first published on 05 May 2015


Article type: Paper
DOI: 10.1039/C5RA02311D
Author version
available:
Download author version (PDF)
Citation: RSC Adv., 2015,5, 42233-42241

  •   Request permissions

    Lanthanum breaks the balance between osteogenesis and adipogenesis of mesenchymal stem cells through phosphorylation of Smad1/5/8

    D. Liu, K. Ge, J. Sun, S. Chen, G. Jia and J. Zhang, RSC Adv., 2015, 5, 42233
    DOI: 10.1039/C5RA02311D

Search articles by author

Spotlight

Advertisements