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Issue 6, 2015
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A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

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Abstract

Herein, we report a simple and homogenous fluorescence method for ultrasensitive DNA detection. It is based on rolling circle amplification (RCA) and fluorescence resonance energy transfer (FRET). As an alternative to a molecular beacon (MB), a linear single-labeled DNA probe was used in this RCA-based fluorescence strategy for DNA detection. The performance of linear DNA probes was compared with that of the MB probe in an RCA-based fluorescence strategy. The results showed that the linear DNA probes could effectively avoid the fluorescence quenching between neighboring signal probes, which would significantly improve the sensitivity of the RCA-based fluorescence strategy. This method exhibited a high sensitivity toward target DNA with a detection limit of 0.7 aM, which was about 100-fold lower than that of the RCA-based fluorescence strategy with MB as the signal probe. This method provides a simple, isothermal, and low-cost approach for sensitive detection of DNA and holds great potential for early diagnosis in gene-related diseases.

Graphical abstract: A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

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Publication details

The article was received on 13 Nov 2014, accepted on 04 Dec 2014 and first published on 05 Dec 2014


Article type: Paper
DOI: 10.1039/C4RA14467H
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RSC Adv., 2015,5, 4019-4025

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    A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification

    F. Zhou, B. Li and J. Ma, RSC Adv., 2015, 5, 4019
    DOI: 10.1039/C4RA14467H

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