A continuous flow microfluidic-MS system for efficient OBOC screening

Abstract

A microfluidic chip based method utilized for effective screening of high-throughput peptide libraries was achieved. Continuous flow bead trapping, sorting, arraying and in situ sequencing was integrated. Peptide library screening with 10⁵ beads was processed within 4 hours and peptide ligands toward the target protein AHA and APN were successfully discovered.