Issue 106, 2014

Immobilised peroxidases from Asparagus acutifolius L. seeds for olive mill waste water treatment

Abstract

This work compares the main enzymatic parameters of a cationic peroxidase (AaP-1-4), purified and characterized from Asparagus acutifolius L. seeds (Mol. Biotechnol., 2014, 56, 738–746) and its immobilised form (Eup-AaP-1-4), on Eupergit® CM with Horseradish peroxidase. The optimum in the pH-activity profile was pH 4.0 and pH 3.0 for AaP-1-4 and Eup-AaP-1-4, respectively. Ca2+ cation enhanced both enzymatic activities, however, when submitted to a temperature stress (120 min at 50 °C) Eup-AaP-1-4 lost only 20% activity while AaP-1-4 70%. Furthermore, AaP-1-4 was proved to be able to remove (poly)phenols in olive mill waste water (OMW), with hydrogen peroxide electron donor. The Eup-AaP-1-4 kinetic proprieties were investigated and the operational stability evaluated in a continuous stirred membrane bioreactor. AaP-1-4 appears to be a novel non-expensive source of peroxidases suitable for biotechnological applications in the environmental field for the removal of aqueous (poly)phenols produced from several industrial processes.

Graphical abstract: Immobilised peroxidases from Asparagus acutifolius L. seeds for olive mill waste water treatment

Article information

Article type
Paper
Submitted
26 Sep 2014
Accepted
07 Nov 2014
First published
07 Nov 2014

RSC Adv., 2014,4, 61482-61490

Author version available

Immobilised peroxidases from Asparagus acutifolius L. seeds for olive mill waste water treatment

V. Guida, E. Niro, N. Landi, A. Chambery, A. Parente, L. Cantarella, M. Cantarella and A. Di Maro, RSC Adv., 2014, 4, 61482 DOI: 10.1039/C4RA11310A

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