Issue 90, 2014

Rapid 18F-radiolabeling of peptides from [18F]fluoride using a single microfluidics device

Abstract

To date the majority of 18F-peptide radiolabeling approaches are multi-step, low yielding and time-consuming processes. Given the short half-life of 18F (109.8 min), it is critical that methods are developed to increase the efficiency of this process with simpler, higher yielding and faster reactions that can be rapidly translated into clinical use. Here, we demonstrate the first microfluidic synthesis of the [18F]F-Py-TFP prosthetic group with radiochemical yields of up to 97% and a synthesis time of 3 min. In addition, we utilized a single microfluidics device to prepare the [18F]F-Py-YGGFL peptide using [18F]F-Py-TFP, from [18F]fluoride in a two-step, fully automated approach. The model peptide NH2-YGGFL was radiolabeled with [18F]F-Py-TFP in up to 28% overall radiochemical yield within 8 minutes starting from anhydrous [18F]fluoride.

Graphical abstract: Rapid 18F-radiolabeling of peptides from [18F]fluoride using a single microfluidics device

Supplementary files

Article information

Article type
Paper
Submitted
16 Sep 2014
Accepted
25 Sep 2014
First published
25 Sep 2014

RSC Adv., 2014,4, 49529-49534

Author version available

Rapid 18F-radiolabeling of peptides from [18F]fluoride using a single microfluidics device

R. C. Cumming, D. E. Olberg and J. L. Sutcliffe, RSC Adv., 2014, 4, 49529 DOI: 10.1039/C4RA10520F

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