Issue 93, 2014
From the journal:

RSC Advances

Development of a prokaryotic-like polycistronic expression system based on a virus-originated internal ribosome entry site (IRES) in industrial eukaryotic microorganisms†

Linlin Xu,‡ab   Gang Wang,‡a   Wenya Wang,b   Xiaofeng Lv,b   Qiang Li*a  and  Aixia Yanb  

* Corresponding authors

a Key Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, China
E-mail: liqiang@tsinghua.edu.cn
Fax: +86-10-62789847

b College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China

Abstract

A prokaryotic-like polycistronic expression system was developed in Pichia pastoris and Acremonium chrysogenum. With simplicity and higher effectiveness, this polycistronic expression system is suitable for introducing multiple genes or entire metabolic pathways into industrial eukaryotic microorganisms.

Graphical abstract: Development of a prokaryotic-like polycistronic expression system based on a virus-originated internal ribosome entry site (IRES) in industrial eukaryotic microorganisms

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Article information

DOI
https://doi.org/10.1039/C4RA07679F
Article type
Communication
Submitted
27 Jul 2014
Accepted
07 Oct 2014
First published
07 Oct 2014

RSC Adv., 2014,4, 51615-51618

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Development of a prokaryotic-like polycistronic expression system based on a virus-originated internal ribosome entry site (IRES) in industrial eukaryotic microorganisms

L. Xu, G. Wang, W. Wang, X. Lv, Q. Li and A. Yan, RSC Adv., 2014, 4, 51615 DOI: 10.1039/C4RA07679F

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