Synthesis of choline sulfonate buffers and their effect on cytochrome c dissolution and oxidation state

Abstract

Seven choline sulfonates with buffering properties were prepared in good yields (74–94%) and high purity by reacting choline hydroxide with different Good's buffers. Choline sulfonate buffers containing hydroxyl group-rich cations appeared to be liquid at room temperature. The dissolution of cytochrome c in the synthesized choline sulfonates was investigated. It was found that complete solubilisation of this heme protein in most of the choline sulfonate buffers can be obtained through addition of 21–31% (w/w%) water. In these hydrated choline sulfonates, cyt c is solubilised in its reduced form.