Jump to main content
Jump to site search

Issue 7, 2014
Previous Article Next Article

Interparticle mesoporous silica as an effective support for enzyme immobilisation

Author affiliations

Abstract

Mesoporous silica materials with cylindrical pores (MPSC) and interparticle pore structures (IPMPS) were synthesized by typical sol–gel methods, and their structural properties were characterized. The enzyme immobilised on IPMPS exhibited higher specific reactivity because of an improvement in the substrate affinity of the enzyme immobilised on the pore spaces of the IPMPS support. Fourier transform infrared and circular dichroism spectroscopy indicated that the highly ordered structure of formaldehyde dehydrogenase (FDH) is not altered by binding to IPMPS and MPSC surfaces. Interestingly, after 10 repeated reactions, FDH immobilised on IPMPS exhibited a residual activity higher than that of FDH immobilised on MPSC. The cycle performance of the enzyme immobilised on MPSC decreased because of support aggregation by the released enzyme. Meanwhile, IPMPS has a high-intensity surface electrical charge that is highly dispersible in the presence of enzymes. In addition, enzymes that were inactive because of being buried inside mesopores are quantitatively determined. The dependency of the activity of FDH immobilised on IPMPS and MPSC as a function of substrate (formaldehyde) concentration was also evaluated to determine the potential application of these materials as biosensors. Formaldehyde concentrations of 3.0–500 μM could be detected using FDH immobilised on the IPMPS support.

Graphical abstract: Interparticle mesoporous silica as an effective support for enzyme immobilisation

Back to tab navigation

Supplementary files

Article information


Submitted
29 Oct 2013
Accepted
02 Dec 2013
First published
03 Dec 2013

RSC Adv., 2014,4, 3573-3580
Article type
Paper

Interparticle mesoporous silica as an effective support for enzyme immobilisation

Y. Masuda, S. Kugimiya, Y. Kawachi and K. Kato, RSC Adv., 2014, 4, 3573
DOI: 10.1039/C3RA46122J

Social activity

Search articles by author

Spotlight

Advertisements