Issue 19, 2013
From the journal:

RSC Advances

Ultra-small fluorescent bile acid conjugated PHB–PEG block copolymeric nanoparticles: synthesis, characterization and cellular uptake

Kiran Chaturvedi,a   Kuntal Ganguly,a   Anandrao R. Kulkarni,*a   Mallikarjuna N. Nadagouda,a   Jennifer Stowbridge,b   Walter E. Rudzinskib  and  Tejraj M. Aminabhavi*a  

* Corresponding authors

a Soniya Education Trust's College of Pharmacy, S. R. Nagar, Dharwad, 580 002, India
E-mail: kulkarni73@yahoo.com, aminabhavit@gmail.com
Fax: +91 836 2467190
Tel: +91 836 2448540

b Department of Chemistry and Biochemistry, Texas State University, San Marcos, Texas, USA
E-mail: wr01@txstate.edu
Tel: +1 512 245 3120

Abstract

A novel method based on solvent-diffusion and solvent evaporation is reported for the first time to prepare ultra-small (10 nm) nanoparticles (NPs) of deoxycholic acid conjugated polyhydroxybutyrate–polyethylene glycol block copolymer (DOCA–PHB–PEG). Cellular uptake of the NPs after attachment of a fluorophore is demonstrated with an aim to enable better understanding of the effect of such a small size on the NPs transport in the cells. The novel DOCA–PHB–PEG copolymer showed low cytotoxic profile and the FITC–insulin labeled ultra-small NPs showed a higher time-dependant cellular uptake. These NPs exhibit an excellent potential in delivering intra-cellular therapeutics.

Graphical abstract: Ultra-small fluorescent bile acid conjugated PHB–PEG block copolymeric nanoparticles: synthesis, characterization and cellular uptake

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Article information

DOI
https://doi.org/10.1039/C3RA22283G
Article type
Paper
Submitted
25 Sep 2012
Accepted
27 Feb 2013
First published
28 Feb 2013

RSC Adv., 2013,3, 7064-7070

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Ultra-small fluorescent bile acid conjugated PHB–PEG block copolymeric nanoparticles: synthesis, characterization and cellular uptake

K. Chaturvedi, K. Ganguly, A. R. Kulkarni, M. N. Nadagouda, J. Stowbridge, W. E. Rudzinski and T. M. Aminabhavi, RSC Adv., 2013, 3, 7064 DOI: 10.1039/C3RA22283G

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