Issue 21, 2013
From the journal:

RSC Advances

Dendrons and dendrimers as pseudochaperonins for refolding of proteins†

Priyanka Dubey,a   Saurabh Gautam,a   P. P. Praveen Kumar,a   Sandhya Sadanandan,a   V. Haridas*a  and  Munishwar N. Gupta*a  

* Corresponding authors

a Department of Chemistry, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India
E-mail: appliedbiocat@yahoo.co.in, h_haridas@hotmail.com
Fax: +91-11-2658 1073
Tel: +91-11-2659 1503

Abstract

Peptide dendrimers are screened for “artificial chaperone” (protein refolding) activity by a sensitive fluorescence based assay. The refolding with largest dendrimer is found to help in recovering biological activity of >90% in the case of unfolded lipases and amylases. The refolding yields decrease down to 14% with a decrease in the complexity and hydrophobicity of the dendron/dendrimer. CD spectroscopy confirms the correct refolding in terms of secondary structure contents of the proteins. The DLS data indicates that presence of the dendrons/dendrimers facilitates protein refolding by preventing the aggregation of proteins.

Graphical abstract: Dendrons and dendrimers as pseudochaperonins for refolding of proteins

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Article information

DOI
https://doi.org/10.1039/C3RA00061C
Article type
Paper
Submitted
07 Jan 2013
Accepted
15 Mar 2013
First published
20 Mar 2013

RSC Adv., 2013,3, 8016-8020

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Dendrons and dendrimers as pseudochaperonins for refolding of proteins

P. Dubey, S. Gautam, P. P. P. Kumar, S. Sadanandan, V. Haridas and M. N. Gupta, RSC Adv., 2013, 3, 8016 DOI: 10.1039/C3RA00061C

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