Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 6, 2012
Previous Article Next Article

Scaffold-free tissue engineering using cell sheet technology

Author affiliations


Cell sheet technology is a tissue engineering methodology requiring no scaffolds. Confluent cultured cells can be harvested as an intact cell sheet using a temperature-responsive polymer, poly(N-isoproplyacrylamide) (PIPAAm), grafted cell culture surface, offering noninvasive control of cell attachment and detachment only by changing the temperature across 32 °C, without any protease treatments. Avoiding protease treatment preserves complete cell–cell junctions, cell surface proteins, and the extracellular matrix in the cell sheet. Therefore, functional three-dimensional (3D) tissue can be easily fabricated by layering cell sheets without the use of scaffolds. Cell sheet technology has been applied in regenerative medicine for several tissues, and a number of clinical trials have already started. In addition, to fabricate more complex and functional 3D tissue, cell micro-patterning technology can be combined with cell sheet technology, and this interdisciplinary technology could produce interesting results. In this review, recent advances in temperature-responsive culture surfaces, cell sheet and cell micro-patterning technologies are summarized and discussed. In addition, the application of these technologies to regenerative medicine and the re-construction of various tissues including heterogeneous tissues are also discussed.

Graphical abstract: Scaffold-free tissue engineering using cell sheet technology

Back to tab navigation

Publication details

The article was received on 09 Sep 2011, accepted on 18 Nov 2011 and first published on 04 Jan 2012

Article type: Review Article
DOI: 10.1039/C2RA00704E
Citation: RSC Adv., 2012,2, 2184-2190

  •   Request permissions

    Scaffold-free tissue engineering using cell sheet technology

    Y. Haraguchi, T. Shimizu, M. Yamato and T. Okano, RSC Adv., 2012, 2, 2184
    DOI: 10.1039/C2RA00704E

Search articles by author