Jump to main content
Jump to site search

Issue 5, 2014
Previous Article Next Article

Synthesis and characterization of variable conformation pH responsive block co-polymers for nucleic acid delivery and targeted cell entry

Author affiliations

Abstract

Responsive materials that change conformation with varying pH have been prepared from a range of amphiphilic block co-polymers. The individual blocks are composed of (a) permanently hydrophilic chains with neutral functionality and (b) acrylate polymers with weakly basic side-chains. Variation in co-monomer content, molar mass and block ratios/compositions leads to a range of pH-responses, manifest through reversible self-assembly into micelles and/or polymersomes. These transitions can be tuned to achieve environmental responses in a pH range from 5–7, as shown by turbidimetric analysis, NMR and dynamic light scattering measurements (DLS). Further characterization by transmission electron microscopy (TEM) indicates that polymersomes with diameters of 100–200 nm can be formed under certain pH-ranges where the weakly basic side-chains are deprotonated. The ability of the systems assembled with these polymers to act as pH-responsive containers is shown by DNA encapsulation and release studies, and their potential for application as vehicle for drug delivery is proved by cell metabolic activity and cell uptake measurements.

Graphical abstract: Synthesis and characterization of variable conformation pH responsive block co-polymers for nucleic acid delivery and targeted cell entry

Back to tab navigation

Supplementary files

Article information


Submitted
06 Jun 2013
Accepted
11 Jul 2013
First published
12 Jul 2013

This article is Open Access

Polym. Chem., 2014,5, 1626-1636
Article type
Paper

Synthesis and characterization of variable conformation pH responsive block co-polymers for nucleic acid delivery and targeted cell entry

T. Matini, N. Francini, A. Battocchio, S. G. Spain, G. Mantovani, M. J. Vicent, J. Sanchis, E. Gallon, F. Mastrotto, S. Salmaso, P. Caliceti and C. Alexander, Polym. Chem., 2014, 5, 1626 DOI: 10.1039/C3PY00744H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Read more about how to correctly acknowledge RSC content.


Social activity

Search articles by author

Spotlight

Advertisements