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Issue 6, 2020
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Effect of linker length on photo-cross-linking position mediated by click chemistry via [2 + 2]photocycloaddition

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Abstract

Ultrafast reversible DNA/RNA photo-cross-linking is a powerful tool for regulating the target strand in living cells. In particular, 3-cyanovinylcarbazole (CNVK) and 3-cyanovinylcarbazole modified by D-threoninol (CNVD) can photo-cross-link to pyrimidine bases within a few seconds of photoirradiation. However, these photo-cross-linkers can only cross-link to the counter base if it is adjacent to the 5′-side (−1 position). In this study, we synthesized novel photo-cross-linkers with varying linker lengths capable of photo-cross-linking with pyrimidine bases at locations other than the −1 position via click chemistry. The photo-cross-linking site was dependent on linker length.

Graphical abstract: Effect of linker length on photo-cross-linking position mediated by click chemistry via [2 + 2]photocycloaddition

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Supplementary files

Article information


Submitted
16 Mar 2020
Accepted
12 May 2020
First published
19 May 2020

Photochem. Photobiol. Sci., 2020,19, 776-782
Article type
Communication

Effect of linker length on photo-cross-linking position mediated by click chemistry via [2 + 2]photocycloaddition

K. Fujimoto, K. Ishida, L. Xue and S. Nakamura, Photochem. Photobiol. Sci., 2020, 19, 776
DOI: 10.1039/D0PP00098A

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