Issue 3, 2020
From the journal:

Photochemical & Photobiological Sciences

Quaternized styryl-azinium fluorophores as cellular RNA-binders†

Valentina Botti, ORCID logo *a   Lorena Urbanelli, ORCID logo b   Krizia Sagini, ORCID logo b   Luigi Tarpani, ORCID logo a   Alessio Cesaretti, ORCID logo a   Cosimo G. Fortuna ORCID logo c  and  Fausto Elisei ORCID logo a  

* Corresponding authors

a Department of Chemistry, Biology and Biotechnology and Center of Excellence on Innovative Nanostructured Materials (CEMIN), University of Perugia, via Elce di Sotto 8, 06123 Perugia, Italy
E-mail: valentinabotti.90@gmail.com
Tel: +39 075 585 5590

b Department of Chemistry, Biology and Biotechnology, University of Perugia, via del Giochetto, 06126 Perugia, Italy

c Department of Chemical Sciences, University of Catania, viale Andrea Doria 6, 95125 Catania, Italy

Abstract

The capability of three quaternized styryl-azinium iodides to bind cellular RNA has been tested by means of Fluorescence Confocal Microscopy imaging of stained MCF-7 cells treated with RNase. Their association constants have been estimated through spectrophotometric and fluorimetric titrations with tRNA and compared to their affinity toward DNA. Transient absorption spectroscopy with femtosecond resolution confirmed the binding of the investigated compounds with tRNA and shed new light on the excited state dynamics of their complexes, by revealing a significant lengthening of the lifetime of S1 upon complexation, which parallels the fluorescence quantum yield enhancement.

Graphical abstract: Quaternized styryl-azinium fluorophores as cellular RNA-binders

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Article information

DOI
https://doi.org/10.1039/C9PP00465C
Article type
Paper
Submitted
25 Nov 2019
Accepted
25 Feb 2020
First published
26 Feb 2020

Photochem. Photobiol. Sci., 2020,19, 362-370

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Quaternized styryl-azinium fluorophores as cellular RNA-binders

V. Botti, L. Urbanelli, K. Sagini, L. Tarpani, A. Cesaretti, C. G. Fortuna and F. Elisei, Photochem. Photobiol. Sci., 2020, 19, 362 DOI: 10.1039/C9PP00465C

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