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Issue 9, 2015
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Optimizing optogenetic constructs for control over signaling and cell behaviours

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Abstract

Optogenetic tools have recently been developed that enable dynamic control over the activities of select signaling proteins. They provide the unique ability to rapidly turn signaling events on or off with subcellular control in living cells and organisms. This capability is leading to new insights into how the spatial and temporal coordination of signaling events governs dynamic cell behaviours such as migration and neurite outgrowth. These tools can also be used to dissect a protein's signaling functions at different organelles. Here we review the properties of photoreceptors from diverse organisms that have been leveraged to control signaling in mammalian cells. We emphasize recent engineering approaches that have been used to create optogenetic constructs with optimized spectral, kinetic, and signaling properties for controlling cell behaviours.

Graphical abstract: Optimizing optogenetic constructs for control over signaling and cell behaviours

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Publication details

The article was received on 24 Apr 2015, accepted on 23 Jun 2015 and first published on 24 Jun 2015


Article type: Perspective
DOI: 10.1039/C5PP00171D
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Photochem. Photobiol. Sci., 2015,14, 1578-1585

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    Optimizing optogenetic constructs for control over signaling and cell behaviours

    P. R. O'Neill and N. Gautam, Photochem. Photobiol. Sci., 2015, 14, 1578
    DOI: 10.1039/C5PP00171D

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