Issue 7, 2015

In vivo evaluation of photodynamic inactivation using Photodithazine® against Candida albicans

Abstract

This study describes the photoinactivation of Candida albicans in a murine model of oral candidosis, mediated by Photodithazine® (PDZ). Six-week-old female Swiss mice were immunosuppressed, and inoculated with C. albicans to induce oral candidosis. After five days, photodynamic inactivation (PDI) mediated by PDZ at concentrations of 75, 100, 125 and 150 mg L−1 was applied on the tongue of mice. Next, microbiological evaluation was performed by recovering C. albicans from the tongue via colony forming units (CFU mL−1). After 24 h of treatment, the animals were killed and the tongues were surgically removed for histological analysis. PDI was effective in reducing C. albicans on the tongue of mice using 100 mg L−1 of PDZ, when compared to the positive control group (without treatment). No adverse effect on the tongue tissue was verified after PDI. Therefore, PDI was effective for inactivation of C. albicans without causing any harmful effects on host tissues, which is promising for future clinical trials.

Graphical abstract: In vivo evaluation of photodynamic inactivation using Photodithazine® against Candida albicans

Associated articles

Article information

Article type
Paper
Submitted
03 Oct 2014
Accepted
16 May 2015
First published
20 May 2015

Photochem. Photobiol. Sci., 2015,14, 1319-1328

In vivo evaluation of photodynamic inactivation using Photodithazine® against Candida albicans

J. C. Carmello, L. N. Dovigo, E. G. Mima, J. H. Jorge, C. A. de Souza Costa, V. S. Bagnato and A. C. Pavarina, Photochem. Photobiol. Sci., 2015, 14, 1319 DOI: 10.1039/C4PP00368C

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