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Issue 7, 2010
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Novel photosensitisers derived from pyropheophorbide-a: uptake by cells and photodynamic efficiency in vitro

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Abstract

Photodynamic Therapy (PDT) is a minimally invasive procedure used for treating a range of neoplastic diseases, which utilises combined action of light and a PDT drug called a photosensitiser. The efficiency of this treatment depends crucially on the properties of the photosensitiser used, namely on its efficient uptake by cells or by the surrounding vasculature, intracellular localisation, minimal dark toxicity and substantial phototoxicity. In this report we compare the spectroscopic properties, cell uptake and in vitro phototoxicity of two novel hydrophilic photosensitisers derived from pyropheophorbide-a (PPa). Both new photosensitisers have the potential to form bioconjugates with antibody fragments for targeted PDT. We find that the photophysical properties of both new photosensitisers are favourable compared to the parent PPa, including enhanced absorption in the red spectral region and substantial singlet oxygen quantum yields. Both molecules show efficient cellular uptake, but display a different intracellular localisation. Both new photosensitisers exhibit no significant dark-toxicity at concentrations of up to 100 μM. The phototoxicity of the two photosensitisers is strikingly different, with one derivative being 13 times more efficient than the parent PPa and another derivative being 18 times less efficient in SKOV3 ovarian cancer cells. We investigate the reasons behind such drastic differences in phototoxicity using confocal fluorescence microscopy and conclude that intracellular localisation is a crucial factor in the photodynamic efficiency of pheophorbide derivatives. These studies highlight the underlying factors behind creating more potent photosensitisers through synthetic manipulation.

Graphical abstract: Novel photosensitisers derived from pyropheophorbide-a: uptake by cells and photodynamic efficiency in vitro

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Publication details

The article was received on 01 Mar 2010, accepted on 27 Apr 2010 and first published on 08 Jun 2010


Article type: Paper
DOI: 10.1039/C0PP00038H
Citation: Photochem. Photobiol. Sci., 2010,9, 1033-1041

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    Novel photosensitisers derived from pyropheophorbide-a: uptake by cells and photodynamic efficiency in vitro

    I. Stamati, M. K. Kuimova, M. Lion, G. Yahioglu, D. Phillips and M. P. Deonarain, Photochem. Photobiol. Sci., 2010, 9, 1033
    DOI: 10.1039/C0PP00038H

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